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Biblioteca (s) : |
INIA La Estanzuela. |
Fecha : |
02/03/2017 |
Actualizado : |
07/03/2017 |
Autor : |
GARCIA, J.P; GIANNITTI, F.; FINNIE, J.W.; MANAVIS, J. .; BEINGESSER, J.; ADAMS ,V.; ROOD, J.I.; UZAL, F.A. |
Afiliación : |
FEDERICO GIANNITTI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
Título : |
Comparative Neuropathology of Ovine Enterotoxemia Produced by Clostridium perfringens Type D Wild-Type Strain CN1020 and Its Genetically Modified Derivatives. |
Fecha de publicación : |
2015 |
Fuente / Imprenta : |
Veterinary Pathology, 2015, v.52.n.6. p.1250-1253, 2015. |
Idioma : |
Inglés |
Contenido : |
Abstract
Clostridium perfringens type D causes enterotoxemia in sheep and goats. The disease is mediated by epsilon toxin (ETX), which affects the cerebrovascular endothelium, increasing vascular permeability and leading to cerebral edema. In the present study, we compared the distribution and severity of the cerebrovascular changes induced in lambs by C. perfringens type D strain CN1020, its isogenic etx null mutant, and the ETX-producing complemented mutant. We also applied histochemical and immunohistochemical markers to further characterize the brain lesions induced by ETX. Both ETX-producing strains induced extensive cerebrovascular damage that did not differ significantly between each other in nature, neuroanatomic distribution, or severity. By contrast, lambs inoculated with the etx mutant or sterile, nontoxic culture medium did not develop detectable brain lesions, confirming that the neuropathologic effects observed in these infections are dependent on ETX production. Lambs treated with the wild-type and complemented strains showed perivascular and mural vascular edema, as well as serum albumin extravasation, particularly severe in the cerebral white matter, midbrain, medulla oblongata, and cerebellum. Brains of animals inoculated with the ETX-producing strains showed decreased expression of glial fibrillary acidic protein and increased expression of aquaporin-4 in the end-feet processes of the astrocytes around blood vessels. Early axonal injury was demonstrated with anti?amyloid precursor protein immunohistochemistry. Perivascular accumulation of macrophages/microglia with intracytoplasmic albumin globules was also observed in these animals. This study demonstrates that ETX is responsible for the major cerebrovascular changes in C. perfringens type D?induced disease. MenosAbstract
Clostridium perfringens type D causes enterotoxemia in sheep and goats. The disease is mediated by epsilon toxin (ETX), which affects the cerebrovascular endothelium, increasing vascular permeability and leading to cerebral edema. In the present study, we compared the distribution and severity of the cerebrovascular changes induced in lambs by C. perfringens type D strain CN1020, its isogenic etx null mutant, and the ETX-producing complemented mutant. We also applied histochemical and immunohistochemical markers to further characterize the brain lesions induced by ETX. Both ETX-producing strains induced extensive cerebrovascular damage that did not differ significantly between each other in nature, neuroanatomic distribution, or severity. By contrast, lambs inoculated with the etx mutant or sterile, nontoxic culture medium did not develop detectable brain lesions, confirming that the neuropathologic effects observed in these infections are dependent on ETX production. Lambs treated with the wild-type and complemented strains showed perivascular and mural vascular edema, as well as serum albumin extravasation, particularly severe in the cerebral white matter, midbrain, medulla oblongata, and cerebellum. Brains of animals inoculated with the ETX-producing strains showed decreased expression of glial fibrillary acidic protein and increased expression of aquaporin-4 in the end-feet processes of the astrocytes around blood vessels. Early axonal injury was demonstrated wi... Presentar Todo |
Thesagro : |
ENFERMEDADES DE LOS ANIMALES. |
Asunto categoría : |
-- |
Marc : |
LEADER 02512naa a2200217 a 4500 001 1056750 005 2017-03-07 008 2015 bl uuuu u00u1 u #d 100 1 $aGARCIA, J.P 245 $aComparative Neuropathology of Ovine Enterotoxemia Produced by Clostridium perfringens Type D Wild-Type Strain CN1020 and Its Genetically Modified Derivatives.$h[electronic resource] 260 $c2015 520 $aAbstract Clostridium perfringens type D causes enterotoxemia in sheep and goats. The disease is mediated by epsilon toxin (ETX), which affects the cerebrovascular endothelium, increasing vascular permeability and leading to cerebral edema. In the present study, we compared the distribution and severity of the cerebrovascular changes induced in lambs by C. perfringens type D strain CN1020, its isogenic etx null mutant, and the ETX-producing complemented mutant. We also applied histochemical and immunohistochemical markers to further characterize the brain lesions induced by ETX. Both ETX-producing strains induced extensive cerebrovascular damage that did not differ significantly between each other in nature, neuroanatomic distribution, or severity. By contrast, lambs inoculated with the etx mutant or sterile, nontoxic culture medium did not develop detectable brain lesions, confirming that the neuropathologic effects observed in these infections are dependent on ETX production. Lambs treated with the wild-type and complemented strains showed perivascular and mural vascular edema, as well as serum albumin extravasation, particularly severe in the cerebral white matter, midbrain, medulla oblongata, and cerebellum. Brains of animals inoculated with the ETX-producing strains showed decreased expression of glial fibrillary acidic protein and increased expression of aquaporin-4 in the end-feet processes of the astrocytes around blood vessels. Early axonal injury was demonstrated with anti?amyloid precursor protein immunohistochemistry. Perivascular accumulation of macrophages/microglia with intracytoplasmic albumin globules was also observed in these animals. This study demonstrates that ETX is responsible for the major cerebrovascular changes in C. perfringens type D?induced disease. 650 $aENFERMEDADES DE LOS ANIMALES 700 1 $aGIANNITTI, F. 700 1 $aFINNIE, J.W. 700 1 $aMANAVIS, J. . 700 1 $aBEINGESSER, J. 700 1 $aADAMS ,V. 700 1 $aROOD, J.I. 700 1 $aUZAL, F.A. 773 $tVeterinary Pathology, 2015$gv.52.n.6. p.1250-1253, 2015.
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INIA La Estanzuela (LE) |
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Biblioteca (s) : |
INIA Treinta y Tres. |
Fecha actual : |
19/08/2015 |
Actualizado : |
23/05/2018 |
Tipo de producción científica : |
Actividades de Difusión |
Autor : |
INIA TREINTA Y TRES.; PROGRAMA NACIONAL DE INVESTIGACIÓN EN ARROZ.; PROGRAMA NACIONAL EN PRODUCCIÓN Y SUSTENTABILIDAD AMBIENTAL.; PROGRAMA NACIONAL EN CULTIVOS DE SECANO. |
Título : |
Arroz-soja: resultados experimentales 2014-2015. |
Complemento del título : |
Jornada anual Arroz-Soja, 2015, INIA Treinta y Tres, Treinta y Tres, UY. |
Fecha de publicación : |
2015 |
Fuente / Imprenta : |
Treinta y Tres, (Uruguay): INIA, 2015. |
Páginas : |
110 p. |
Serie : |
(INIA Serie Actividades de Difusión; 748) |
ISSN : |
1688-9258 |
Idioma : |
Español |
Thesagro : |
ARROZ; ARROZ ROJO; CONTROL DE ESCARDA; ECONOMIA; ENFERMEDADES DE LAS PLANTAS; FERTILIZACION; FITOMEJORAMIENTO; MANEJO DEL CULTIVO; RIEGO; ROTACIONES; SEMILLAS; SOJA; SUSTENTABILIDAD. |
Asunto categoría : |
F01 Cultivo |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/4928/1/Ad-748.pdf
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Marc : |
LEADER 00961nam a2200325 a 4500 001 1053260 005 2018-05-23 008 2015 bl uuuu u00u1 u #d 022 $a1688-9258 100 1 $aINIA TREINTA Y TRES. 245 $aArroz-soja$bresultados experimentales 2014-2015. 260 $aTreinta y Tres, (Uruguay): INIA$c2015 300 $a110 p. 490 $a(INIA Serie Actividades de Difusión; 748) 650 $aARROZ 650 $aARROZ ROJO 650 $aCONTROL DE ESCARDA 650 $aECONOMIA 650 $aENFERMEDADES DE LAS PLANTAS 650 $aFERTILIZACION 650 $aFITOMEJORAMIENTO 650 $aMANEJO DEL CULTIVO 650 $aRIEGO 650 $aROTACIONES 650 $aSEMILLAS 650 $aSOJA 650 $aSUSTENTABILIDAD 700 1 $aPROGRAMA NACIONAL DE INVESTIGACIÓN EN ARROZ. 700 1 $aPROGRAMA NACIONAL EN PRODUCCIÓN Y SUSTENTABILIDAD AMBIENTAL. 700 1 $aPROGRAMA NACIONAL EN CULTIVOS DE SECANO.
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